Antimicrobial effect of chitosan and propolis membranes on bacteria of dental interest
DOI:
https://doi.org/10.54139/odousuc.v23i1.419Keywords:
chitosan, propolis, antibacterial compounds, membranesAbstract
The health area is constantly exploring new options for infection control, with natural products being a great alternative. Chitosan and propolis are currently widely used in dentistry, with favorable results. Hence, it was proposed to determine, through an explanatory investigation with an experimental laboratory design, the antibacterial effect of a chitosan and propolis membrane on pathogens of dental interest. The preliminary antibacterial activity of 45% propolis dye (TP45%) on Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa was determined using the modified agar diffusion technique with wells, subsequently the minimum inhibitory concentration (MIC) was determined by macrodilution in tubes. and the chitosan/propolis membranes were prepared, which were evaluated by the agar diffusion method in triplicate, using 0.12% chlorhexidine gluconate as a control. S. aureus was more susceptible than E. faecalis, no inhibitory effect on P. aeruginosa was observed. The MIC for S. aureus was 16 ug/mL while for E. faecalis it was 128 ug/mL. TP45% showed activity against Gram positive bacteria, however, the effect was not preserved in chitosan/propolis membranes.
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